B1B cells are treated with demecolicine and released at time zero. Demecolicine halts the cells in mitosis, 60% of the cells revealed mitotic chromosomes. This means that 40% of the cells visible by light microscopy are possibly no longer productive (data not shown). At 4 hours, synchronous cell divisions occur for about one hour. At 5 hours cells should start their G1 phase. The blue dots line indicate the 3HT uptake into the cells, revealing continuous linear increase. The red sqares indicate the incorporation of 3HU into the RNA, revealing a continuous linear increase. The green triangles indicate 3HT incorporation into DNA. Incorporation starts immediately after mitosis, thus allowing replication. Double tip arrows show the standard deviation of values. Two independent samples are taken for each result. The experiment is performed three times with similar results.
