a. The effects of each single treatment (erlotinib, celecoxib and ionizing radiation) and the combinations on COX-2 and actin were assessed by immunoblotting at 20 minutes and 72 hours after treatment in SQ20B, SCC61 and SCC25. Differences in protein levels were assessed by densitometry and are shown in Supplemental figures.
b. The effects of each single treatment (erlotinib, celecoxib and ionizing radiation) and the combinations on PGE-2 concentration assessed by enzyme-linked immunosorbent assay in SQ20B, SCC61 and SCC25.
