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| Figure 2: (a) Schematic illustration of the functional elements of pEPI: Expression of the respective reporter- or transgene (green), driven from an active promoter (turquoise) has to run into the S/MAR element (blue). When these preconditions are not given, S/MAR based vectors fail to establish episomally. In consequence they got lost from the cell or, in rare cases, integrate into the cellular genome. (b) Modifications of pEPI vectors solely apply to promoter, transgene and backbone. |
