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| Figure 5: a. Stimulation of CK (lower panel) and DNA (upper panel) in prOb and poOb with E2 (30nM), D, G, BA and Ral (3000nM) and DPN (420nM) or PPT (390nM). Cells were obtained, cultured, treated and assayed as described. Results are means ± SEM for triplicate cultures from 5 specimen/group. Control means were 33.6±6.8 for prOb and 23.8+2.0nmol/ min/mg protein for poOb for CK. Control means were 3580+215 for prOb and 2880+251 for poOb for DNA. Experimental means compared to control means: *, P <0.05; **, P <0.01. #P<0.05 compared with the level in prOb. b. The effect of pre-treatment for 3 days of prOb and poOb with JKF (1nM) and then treated for 24h with E2 (30nM), D, G, BA and Ral (3000nM) or c. carboxy derivatives (300nM). Cells were obtained, cultured, pre-treated and assayed as described. Control means were 30.6±3.8 for prOb and 22.8+1.5nmol/min/mg protein for poOb. Results are means ± SEM for triplicate cultures from 5 specimen/group. Experimental means compared to control means: *, P <0.05; **, P <0.01; P<0.001. #P<0.05; ##P<0.01 compared with prOb. |
