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| Figure 5: QRT-PCR and In situ hybridization showing cell type-specific expression of Cdc2a mRNA in the Mus musculus uterus following DHT treatment. The lower left panel displays the QRT-PCR mRNA measurements in the whole uterine tissue at 3h, 12h and 24h as well as in the control (Ctl: 24h vehicle-treated). Results are expressed in percent of the control (here defined as 100%) as mean and standard deviation (SD). The remaining panels display the mRNA labelling using the antisense probe of the section through the mouse uterus demonstrating the higher increase of Cdc2a expression as well as the corresponding control section (0h). AS: Antisense probe; S: Sense probe; LE: Luminal epithelial cells; GE: Glandular epithelial cells; S: Stromal cells. Using the sense probe, only a diffuse background is observed. Exposure, 30 days, × 600. |
