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| Figure 1: Biglycan synthesised byASMCs from ApoE-/- mice and ApoE+/+ differs in size. (A) Measurement of radiolabel incorporation into proteoglycans. ASMCs isolated from from ApoE+/+ and ApoE-/- mice mice were treated with and without PDGF (50 ng/ml) in the presence of [35S]-SO4 (50 μCi/ml) for 24 hr. Media containing secreted biglycan was blotted onto chromatography paper and precipitated via cetylpyridinium chloride. Results are expressed as the mean ± S.E.M of normalized data, ##P<0.01 untreated control samples, ** P < 0.01 PDGF treatments using a one-way ANOVA. (B) PDGF stimulates an increase in biglycan size. Secreted proteoglycans from ASMCs from ApoE+/+ and ApoE-/- mice treated as detailed above were isolated using ion exchange chromatography (DEAE Sephacel) and concentrated by ethanol precipitation. Proteoglycans were separated on a 10% polyacrylamide gel. Gel was performed twice from two separate experiments, with a representative shown. |
