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| Figure 1: FBPase, MDH2, Icl1p, Pck1p, GAPDH, and Cpr1p are in the periplasm. (A), Total lysates from wild-type cells expressing Icl1p-HA and Pck1p- Myc were examined for the specificities of antibodies by Western blotting. These antibodies reacted with a single band at the predicted molecular weights for Sec28p, FBPase, MDH2, Icl1p-HA, Pck1p-Myc, GAPDH, and Cpr1p. (B), Wild-type cells expressing Icl1p-HA and Pck1p-Myc were grown in low glucose for 3 days and fixed. Cells were processed and embedded in LR White resin. Thin sections of cells (10 nm) were incubated with or without primary antibodies against Sec28p, FBPase, MDH2, Icl1p-HA, Pck1p-Myc, GAPDH, and Cpr1p in whole cells. Thin sections were then incubated with secondary goat anti-rabbit antibodies conjugated with 10 nm gold particles. Enlargements of the periplasm of the whole cells are shown. PM: Plasma membrane; CW: Cell wall; Bars: 200 nm. (This figure is in a manuscript published in Communicative and Integrative Biology, 2013 [76]). |
