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| Figure 5: The extraction protocol confirms the presence of FBPase, MDH2, Icl1p, Pck1p, GAPDH, and Cpr1p in the extracellular fraction. Wildtype cells expressing Scw4p-GFP were grown in YPKG containing low glucose for three days and extracellular proteins were extracted. The distribution of Scw4p-GFP in the intracellular and extracellular fractions was examined by Western blotting using anti-GFP antibodies. Wild-type cells co-expressing Icl1p-HA and Pck1p-Myc were grown in low glucose media for 3 days and extracted. The distribution of Sec28p, FBPase, MDH2, Icl1p-HA, Pck1p- Myc, GAPDH, and Cpr1p in the intracellular and extracellular fractions was examined by Western blotting using anti-Sec28p, anti-FBPase, anti-HA, anti- Myc, anti-GAPDH and anti-Cpr1p antibodies. (This figure is in a manuscript published in Communicative and Integrative Biology, 2013 [76]). |
