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| Figure 6: Decrease in the percentage of CD19+ cells in spleen of female PFNA-treated mice. Unfractionated splenocytes were washed, incubated with fluorochromeconjugated antibodies and analyzed by flow cytometry. A representative histogram of CD19+ B cells is depicted from VH-treated (shown in black) and PFNA-treated (shown in red) (A) male and (B) female mice. (C) Graphical representation of CD19+ B cell percentage with respect to the whole splenocyte population. The cells were gated on those that were CD4-CD8-. The data are presented as mean ± SE. * denotes p<0.05 as compared to the vehicle (VH) control for the same gender. † denotes p<0.05 as compared between genders for the same treatment group. |
