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Figure 7: Decrease in the percentage of CD14+ cells in spleen of male and female PFNA-treated mice. Unfractionated splenocytes were washed, incubated with fluorochrome-conjugated antibodies and analyzed by flow cytometry. The cells are gated on the CD4-CD8-CD19- population. A representative histogram of CD14+ cells is depicted from VH-treated (shown in black) and PFNA-treated (shown in red) (A) male and (B) female mice. (C) Graphical representation of CD14+ cell percentage with respect to the whole splenocyte population. The cells were gated on those that were CD4-CD8-CD19-. The data are presented as mean ± SE. * denotes p<0.05 as compared to the vehicle (VH) control for the same gender. † denotes p<0.05 as compared between genders for the same treatment group. |