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| Figure 1: Demonstration ofautocrine IGF2/IGF1R activity in GEO colon cancer cells. A-B: RNA was collected from confluent (CONF.) or quiescent day 5 (QUI.) GEO cells and a two-step reverse transcription-PCR procedure were performed. Linear data normalized to the level of confluent GEO (GEO CONF.) cells from either IGF1R or IGF2 quantitative PCR (mean ± S.E.M.; n=3). GAPDH- JOE probe was used as an endogenous control. C: Confluent and quiescent GEO cells were harvested and subjected to western blot analysis. The blots were probed with phospho-IGF1R, IGF1R and actin (as a loading control) antibodies. |
