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| Figure 5: Increased apoptosis in PQIP treated tumor tissues. BALB/c nude mice orthotopically implanted with GEO were treated on days 8-30 post-implantation with either 75mg/kg PQIP or vehicle control by oral gavage. At necropsy, tissues were formalin fixed and paraffin embedded for H&E staining and IHC. A: Apotag TUNEL assay (Oncor) demonstrates increased numbers of apoptotic cells in tumors treated with PQIP compared to control animals. Representative examples of H&E staining and TUNEL assays are shown. B: The apoptotic rate of control and PQIP treated tumor samples was determined by counting positive nuclei in three random 75μm2 fields at 20x magnification whereas proliferation on ki67 assays was equivalent. |
