| |
Primer sequence |
Annealing temperature |
| MMP-3 |
Forwardprimer:5TCTTCAAGGACCTACCTCAGGC-3
Reverseprimer:5GCTAAGGCAAAGCTGCTAGGTc-3 |
60°C |
| IL-10 |
Forwardprimer:5 -AGTTGGACAACAAACCCTGC -3
Reverseprimer:5- AAGCCCAAAGTCCATCAGTG -3 |
56°C |
| COMP |
Forwardprimer:5 - ATGGGAGCAAGTCAGTGGAC -3
Reverseprimer:5- TTGAGGTAGCTGCACTGTGG -3 |
65°C |
| TNF alpha |
Forward primer :
5’-GGCAGGTCTACTTTGGAGTCATTGC-3’-
Reverse primer:
5’-ACATTCGGGGATCCAGTGAGCTCCG-3’ |
60°C |
| β-actin |
Forward primer :
5- ACTGCCGCATCCTCTTCCTC - 3
Reverse primer:
5- ACTCCTGCTTGCTGATCCACAT -3 |
55°C |
