|
| Figure 2: Deletion of yeast mannosyltransferases increased the extracellular activity (a, b) and decreased the glycosylation (c) of Tr -Cel7A. (a, b) The extracellular activity was assayed with culture supernatants using pNPC as the substrate. The definitions of the “Control” and “ Wild type” were shown in Table 1. Data shown are the mean values (± standard error) obtained from three independent experiments. The differences between the modified strains and wild type were all significant (P0.05), even more significant (P<0.01) at 48h. (c) The secreted proteins were precipitated from culture supernatants at 48 h. The Tr -Cel7A proteins were detect by western blot with anti-Flag monoclonal antibody. |
