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| Figure 3: Deletion of yeast mannosyltransferases increased the specific activity and secretion yield of Tr -Cel7A. (a) The purified Tr -Cel7A protein by the Q Sepharose column was used for tested its specific activity with pNPC as the substrate. (b) The secretion yield was detected by ELISA assay with anti-Flag antibody. The samples were secreted protein precipitated from culture supernatants at 48 h. All data shown are the mean values (± standard error) obtained from three independent experiments. The differences between the modified strains and wild type were all significant P<0.01 (**). |
