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| Figure 4: The heterogenous α-1,2-mannosidase Tr -Mds1p decreased the glycosylation (a) and increased the extracellular activity (b) of Tr -Cel7A. (a) The secreted proteins were precipitated from culture supernatants at 48 h. The Tr -Cel7A proteins were detect by western blot with anti-Flag monoclonal antibody. (b) The extracellular Tr -Cel7A activity was assayed with culture supernatants using pNPC as the substrate. The definitions of the “Control” and “ Wild type” were shown in Table 1. All data shown are the mean values (± standard error) obtained from three independent experiments. The differences between the modified strains and wild type were all significant (P<0.05), even more significant (P<0.01) at 48h. |
