| Study |
Participants |
Design |
Outcome |
| Animal studies demonstrating changes in irisin after exercise |
| Bostrom et al. [19] |
5 12-week old B6 mice |
1. 3 weeks of free wheel running
2. 10 days of swim training |
1. Irisin increases by 65% in mice after exercise
2. Injection of antiFNDC5 antibodies prior to exercise reduces induction of UCP1 |
| Brenmoehl et al. [31] |
Acute: 12 70-day old male DUhTP mice
Long-Term: 12 DUhTP and 11 Coeval male mice |
Acute: Submaximal treadmill test, with animals sacrificed immediately after.
Long-Term: Three weeks of free wheel running or kept sedentary (coeval mice), sacrificed at 70 days old. |
Acute: Mice had 1.9-fold higher serum irisin after treadmill test, with higher irisin protein in femoral muscle homogenate. No effect on FNDC5 mRNA
Long: no effect on serum irisin, muscle FNDC5 mRNA/protein |
| Fain et al. [32] |
5 exercised and 8 sedentary Rapacaz FHM pigs (defective LDL receptor)
8 exercised and 8 sedentary normal Yucatan pigs |
Exercised pigs did 16-20 weeks exercise training of daily moderate treadmill training. Samples taken 24hrs after final exercise. |
Plasma irisin rose by 42% in exercised FHM pigs, but not in normal pigs.
No effect on muscle FNDC5 mRNA |
| Animal studies showing no change in irisin after exercise. |
| Seo et al. [33] |
Male Sprague Dawley Rates (n=5-7/group) |
Mice fed either high or low fat diet.
Exercised rats performed 4 weeks of 5day/week treadmill exercise.
Age garlic extract (AGE) groups received 4 weeks AGE injections |
AGE and exercise alone and in combination decrease weight and improve insulin resistance.
Neither intervention alters serum irisin or skeletal muscle FNDC5. |
