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| Figure 7: a. The effect of pre-treatment for 3 days of prOb and poOb with JKF (1nM) and then treated for competitive intracellular E2 binding with E2 (30nM), D, G, BA and Ral (3000nM) or b. E2-Ov, cG-Ov, cD-Ov and cBA-BSA (300nM) or Ral (3000nM) for membranal binding. Cells were obtained, cultured, pre-treated and assayed as described. Results are means ± SEM for triplicate cultures from 5 specimen/group. Experimental means compared to control means: *, P <0.05. #P<0.05 for the hormones after JKF and hormones after vehicle. |
