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Figure 1: Amplified PCR product of 151bp of Nanog analyzed on (1.5%) agarose gel visualized after ethidium bromide stained. Lane M-100 bp marker; Lane 6 is showing up regulation (over expression); lane 1 and 5 showing complete disappearance/deletion of band; lane 3 and 4 have down regulation, while lane 2 showing normal appearance of Nanog3. The present study repeated three times to confirm the appearance of bands with respect to controls. |