| Gene |
Accession number |
Primer sequence |
Amplicon length (bp) |
Amplification efficiency,
E (SE) |
R2 |
KCNN3
variant 1+2 |
NM_002249
NM_170782 |
F-CGTGTCTGTGAAAGGTACCATGACC
R-CACCACGGCCACCACAAGGG |
192 |
1.90 (0.01) |
0.988 |
KCNN3
variant 2 |
NM_170782 |
F- CGCGACATCTGCAAGGACAGT
R- AGAGCTGGACTTCACGTGTGTG |
166 |
- |
- |
KCNN3
|
NM_001204087 |
F-GACCGTCCGTGTCTGTGAAA *
|
71 |
1.83 (0.02) |
0.984 |
| variant 3 |
7 |
R-GGTACCAAGCAGGAAGTGATGAG * |
|
|
|
| TOP1 |
NM_003286 |
PrimerDesign Ltd |
- |
1.78 (0.01) |
0.996 |
| SDHA |
NM_004168 |
PrimerDesign Ltd |
- |
1.83 (0.01) |
0.993 |
SDHA: succinate dehydrogenase complex; KCNN3: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3 (SK3 protein); TOP1:
Topoisomerase (DNA) I
* Primer sequence published by Wittekindt et al. [22]
R2, correlation coefficient of the slope of the standard curve |
