| Primers |
Primer sequences (5’.....3’) |
Length (bp) |
Amplicon size (bp) |
Annealing temp. °C |
| DF53F |
TATCCAACCTCCCGCATAC |
19
19
19
20
20
18 |
570 |
62.5
62.5
63.5 |
| DF606R |
CATTTCACCGCTACACCAC |
| DF113F |
ATCTCAAGAGCACATGCAA |
380 |
| DF472R |
AATAAATCCGGATAACGCTC |
| DF418F |
ACGAATAAGCATCGGCTAAC |
210 |
| DF609R |
AAGCATTTCACCGCTACA |
|
