Figure 5: Transfection of DENV2 viral RNA into Huh-7 cells. Cells were either (A) pre-treated or (B) post-treated with different concentrations of emetine and transfected with equivalent amounts of purified DENV2 RNA. Three days after transfection, the cells were processed for antigen expression by immunoflourescence assay. The data are representative of three independent experiments. Cells pre-treated with emetine did not inhibit the DENV2 infection upon transfection of DENV2 RNA. However, cells post-treated with emetine showed drastic reduction in DENV2 replication. We can hence deduce that inhibitory action of emetine occurs after the release of viral genome into the cytoplasm.