Figure 8: Ultrastructural analysis of DENV2-infected and emetine treated Huh-7 cells. (A, B) DENV2-infected Huh-7 cells treated with 0.1 % of DMSO. (A) DENV2 induced membranous structures and replication complexes (arrows) can be observed. DENV2 particles (arrowheads) can also be observed in close association with the replication complexes (arrow). (B) DENV2 particles (arrowheads) are observed to accumulate within the ER lumen in close association with the virus replication complexes (arrows). (C) DENV2-infected Huh-7 cells that were treated with 0.5 µM of emetine. Distinct membranous structures induced by DENV2 infection are not obvious even though there is extensive proliferation of the ER membranes (arrows). (D) Mock-infected Huh-7 cells that were treated with 0.5 µM of emetine. These cells exhibited typical ultrastructral morphology of non-infected cells.