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| Figure 1: Effect of A23187 and thapsigargin addition on henipavirus replication in vitro. Inhibition of HeV and NiV replication as measured by viral nucleocapsid protein expression determined by immunoassay. Each compound was incubated with cells for 60 minutes, media was then removed and virus inoculum (1,000 TCID50/ml NiV) added and incubated for 60 minutes, then inoculum was removed and replaced with EMEM-10 and cells were incubated overnight. Viral protein was immunoassayed as previously described (Aljofan et al., 2008). Values are expressed as the mean +/- S.E. (n=3). In parallel, cell cytotoxicity assays were performed to determine the relative toxicity of each compound as described in the materials and methods. Non-linear regression analysis was performed using GraphPad Prism. |
