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| Figure 2: Effect of modulators of intracellular calcium release on the efficacy of A23187 and thapsigargin inhibition of henipavirus replication. Vero cells were pretreated for 60 minutes with 10 μM BAPTA, 10 μM EGTA, 5 mM calcium chloride, 10 μM xestospongin C, 1 μM ryanidine or 10 μM verapamil prior to the addition of 10 nM A23187 or 100 nM thapsigargin. Cells were infected, incubated and immunoassayed as above. Values are expressed as the mean +/- S.E. (n=3). |
