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| Figure 4: Expression of IL-8 and TNF- in response to antiviral addition to vero cells. Vero cells were treated overnight with 10 μM 4-hydroxytamoxifen, 10 μM EIPA, 1 μM A23187, 10 μM cyclosporin, 1 μM gliotoxin, 10 μM mibefradil, 1μM neomycin, 100 μM nifedipine, 100 nM PKC inhibitor, 1 μM PMA, 10 μM ribavirin, 100 nM thapsigargin or 100 μM verapamil. Media was then removed and RNA extracted using a Qiagen RNEasy kit as per the manufacturer’s instruction. IL-8 and TNF- expression was quantified following RNA transcription and real time PCR employing SYBR green as described in the materials and methods. Fold change in mRNA transcripts over mock levels were determined using the delta-Ct method. Results are expressed as the mean +/- S.E. (n=3 replicate determinations of each of 3 biological replicates). |
