Primer   Positiona Sequenceb (5' to 3') Cb-3L antisense 3'-end (3919-3904) CGGGCGGGGAGACTCC P5BamT7-1 sense 5'-end (1-13) GAGGATCCTAATACGACTCACTATAGCGATAAACTTAGC P2489 sense 2489-2510 CCATTTTTGACTTCTTTCCCCC P2633 antisense 2633-2609 TGGGTTTTACTGCTGTCGTGTGTAG P3LSma antisense 3'-end (3919-3904) ATATCCCGGGCGGGCGGGGAGACTCC PBamT7-3.1 sense 827-850 GAGGATCCTAATACGACTCACTATAGCACACCCTTACCTCAAAGAGAAAG PBamT7-1.6 sense 2285-2308 GAGGATCCTAATACGACTCACTATAGAATCATGGAGCGCGAACATCCCAC PBamT7-1.3 sense 2513-2538 GAGGATCCTAATACGACTCACTATAGTGAATATCCCTAACATAAACCAATCG P3925 antisense 3502-3479 CCAAACTACCAGCCATTTACCCTC P517 antisense 979-956 CGTAGAAAACCCGCTCCAGTAATC P2527 antisense 2633-2609 TGGGTTTTACTGCTGTCGTGTGTAG P2168 antisense 2992-2971 GGGGCTGTATTGACCTGGATTC Oligo-dT antisense Poly(A) GGCCACGCGTCGACTAGTAC(T)17 Oligo-dG antisense Poly(C) TACTACTCATATATTAATATA(G)14 aBinding position on complete genome sequence of Calibrachoa mottle virus (CbMV, GQ244431) bShaded sequences are not from CbMV and were included to generate restriction sites BamHI or SmaI (bold) and to incorporate T7 RNA polymerase promoter (underlined)

Table 1: Oligonucleotide sequences of primers used for reverse transcription and polymerase chain reaction.

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