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| Figure 2: Competitive inhibition of gp120 binding to determine assay specificity. DC-SIGN was exposed to decreasing concentrations (500 - 0.5 nm) of native gp120, followed by the immediate addition of FITC-gp120. Positive and negative controls were demonstrated by the exposure only to FITC-gp120 or to assay buffer, respectively. Gradual increase of FITC-gp120 binding occurred as the concentration of native gp120 was lowered, showing the specificity of the assay. Data represent mean fluorescence intensity ± S.E.M from an experiment performed in duplicate. Student’s t test was used to determine the statistical significance of a sample for p value = 0.05 and is denoted by an asterisk (*). |
