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| Figure 3: Optimization for DMSO tolerance. DC-SIGN was introduced to decreasing concentrations (2.0% - 0.02%) of DMSO to determine if DMSO had any effect on gp120 binding to DC-SIGN. FITC-gp120 was added directly after DMSO. Positive and negative controls were demonstrated by exposure only to FITC-gp120 or to assay buffer, respectively. The results showed that DMSO had no effect on gp120 binding because none of the DMSO had statistically different readings from the positive control. Data represent mean fluorescence intensity ± S.E.M. from an experiment performed in duplicate. |
