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| Figure 4: Assay validation through screening known DC-SIGN and gp120 inhibitors 507 (20µg/mL), SC-20 (20µg/mL), GN (10µg/mL), HHA (10µg/ mL), NP (10µg/mL), 2G12 (10µg/mL), SI #1 (100µM), and SI #2 (100µM). All inhibitors were combined with DC-SIGN and then followed by FITCgp120. Incubation occurred at 4°C for 1 hour. Positive and negative controls were demonstrated by the exposure to only FITC-gp120 or to assay buffer, respectively. All of the samples except GN showed at least a 66% decrease in fluorescence, indicating that inhibition of DC-SIGN/gp120 binding had taken place. GN had only a 33% decrease, which was calculated to be insignificant. Data represent mean fluorescence intensity ± S.E.M from an experiment performed in triplicate. Student’s t test was used to determine the statistical significance of a sample for p value = 0.05 and is denoted by an asterisk (*). |
