Table 1 | PCR primers used for sequencing of env ..

Gene region	Outer primer, RT-PCR^a
Gene region	Forward	Reverse
gp120	5′ TGTGTGGTCCATAGTAATCATAGAATA ′3	5′ CTCAATAGCCCTCAGCAAATT ′3
	5′ CAATTGGAGAAGTGAATTATAT ′3	5′ CTGGCTCAGCTCGTCTCATTC ′3
	Inner primer, Sequencing-PCR
	Forward	Reverse
gp41	5′ GAAAGAGCAGAAGACAGTGGCAATGA ′3	5′ ATTGGCTCAAAGGATACCTTTGGACA ′3
	5′ CAATATCAGCACAAGCATAAGAGGTAA ′3	5′ TCCTGAGGATTGCTTAAAGATTATTGT ′3
	5′ GGAAATATGAGACAAGCACATTGTAAC ′3	5′ TACCAGACAATAATTGTCTGGCCTGTA ′3
	5′ AAGAGAAGAGTGGTGCAGAGAG ′3	5′ GCCTAACTCTATTCACTATAGA ′3
	5′ TATTGGAATTAGATAAATGGGC ′3	5′ GCCATCCAATCACACTACTT ′3

^aReverse transcriptase PCR reaction for generation of cDNA and elongation

Table 1: PCR primers used for sequencing of env gene of HIV-1.

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