|
| Figure 3: Protection of gene-modified HIV target cells expressing sscFvPRO140 against R5, but not X4 HIV infection. U373-MAGI-CCR5E and U373-MAGICXCR4 CEM cells were transduced with LVX (U373-MAGI-CCR5E/Control and U373-MAGI-CXCR4CEM/Control) or LVX- sscFvPRO140 (U373-MAGI-CCR5E/ sscFvPRO140 and U373-MAGI-CXCR4CEM/sscFvPRO140) vector particles. The transduced U373-MAGI-CCR5E and U373-MAGI-CXCR4CEM cells were subsequently infected with R5 HIVJRFL or X4 HIVHXB2 Env-pseudotyped reporter vector particles encoding dsRed, respectively. Cells were analyzed by fluorescence microscopy. Representative images are shown. White arrows indicate infected gene-modified cells (dsRed+ZsGreen+); level of genemodification ˜30%; scale bar = 25 μm. |
