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| Figure 4: Protection of gene-modified HIV target cells by sscFvPRO140. U373- MAGI-CCR5E cells were transduced with LVX (U373-MAGI-CCR5E/Control) or LVX-sscFvPRO140 (U373-MAGI-CCR5E/sscFvPRO140) vector particles. Transduced cells were infected with R5 HIVJRFL Env-pseudotyped reporter vector particles expressing dsRed; n=2. (a) Cells were analyzed by flow cytometry. (b) Infected gene-modified cells (ZsGreen1+dsRed+) were quantified to determine relative infection compared to control cells. (c) Cells were analyzed by fluorescence microscopy; level of gene-modification ˜95%; scale bar = 50 μm. |
