Table 1 | Characterization and Identification of the isolated virus..

Species	Samples from scabs	Isolation virus on				Identification of isolated virus
		CAM		Vero		IFAT	ELISA	PCR
		No	%	No	%	IFAT	ELISA	PCR
sheep	15	3	20	2	13.33	+	+	+
goat	15	4	26.6	1	6.66	+	+	+
human	9	1	11.11	0	0	+	+	+
Positive control ORF virus	1	+	+	+	+	+	+	+

Table 1: Characterization and Identification of the isolated virus comparing with positive control ORF virus by FAT, ELISA and PCR.
+ = positive result
Data presented in Table 1 showed clearly that isolation of Orf virus on ECE (SPF and commercial) from prepared skin lesions (nodules, pustules, scabs and hand biopsy) from sheep , goats and human at different localities. The results showed that the number of the clinical samples that develop positive pathological changes on CAM of ECE after the third passage were 3 samples out 15 (20%),4 samples out 15 (26.66%) and 1 samples out 9 (11.11%)respectively for sheep, goats and human clinical specimens. All samples from each of both positive and negative results on ECE were inoculated on confluent sheet of Vero cell culture. All samples which gave positive results after the third passage were two samples out fifteen (13.33%), one samples out fifteen (6.66%) and Zero samples out nine (0%) respectively gave positive results with inoculation on MDBK cell culture after the third passage. The developed CPE on Vero cells 5-7 days post inoculation appeared in the form of cell rounding, multinucleated cells, then progressing of the CPE till distortion of the monolayer and cell detachment. The Vero cell line was less sensitive than ECE for isolation and propagation of Orf virus. Identification and confirmation of isolated Orf virus was done on the molecular and biological levels. Harvested inoculated ECE positive results showed clear by FAT, ELISA, AGPT and PCR.

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