Location Species Sera Protein A ELISA IFAT AGPT Mean +ve  ± SD %  Mean +ve  ± SD % Mean +ve  ± SD % El wasta city sheep 15 6 ± 0.54A 40 4 ± 0.58 A 26.67 3 ± 0.57 A 20 goat 12 4 ± 0.57 A 33.33 3 ± 0.57 A 25 2 ± 0.33 A 16.67 human 11 1 ± 0.00 9.09 1 ± 0.00 9.09 0 ± 00 0 Nasar city sheep 20 8 ± 0.54 A 40 6 ± 0.58 A 30 3 ± 0.00 15 goat 8 3 ± 0.33 37.5 2 ± 0.01 25 1 ± 0.00 12.5 human 15 2 ± 0.00 13.33 1 ± 0.00 6.66 1 ± 0.00 6.66 Baba city sheep 13 4 ± 0.58 A 30.77 2 ± 0.00 15.38 5 ± 0.58 A 38.46 goat 9 2 ± 0.00 22.22 1 ± 0.00 11.11 1 ± 0.00 11.11 human 13 1 ± 0.00 7.6 1 ± 0.00 7.6 0 ± 0.00 0 Total  ± SD 116 31 ± 0.57 A 26.75 21 ± 0.58 A 18.10 16 ± 0.58 A 13.7

Table 2: Examination of serum samples collected from sheep, goat and human by different serological test in different localities at Beni -Suef Governorate Egypt A: Significant at P<0.05 SD: Stander Division Table 2: showed clearly that the total of 116 serum samples were tested for the prevalence of anti Orf virus antibody by using different serological test protein A-ELISA, IFAT and AGPT. The percentage of positive serum samples for anti Orf virus antibody by protein-A-ELISA was (37.5, 31.03 and 10.26), IFAT were (31.03, 20.59 and 7.69) and AGPT were (10.26, 17.24 and 2.56) in sheep, goat and human in Beni-Suef Governorate respectively. We used triplicates of serum samples tested.

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