Time Log10 ORFV  titer kept at 4°C  Log10 ORFV  titer kept at 37°C and 56°C Log10 ORFV titer  was treated with essential oil (v/v) Log10 37°C Log10 56°C Log10  titer Reduction index 0 min 5.9 5.9 5.9 5.6 0.3 10 min 5.9 5.4 5.4 3.5 2.4 20 min 5.8 5.0 2.5 2.5 3.4 30 min 5.8 4.8 0 1.6 4.2 40 min 5.6 4.2 0 1.2 4.4 50 min 5.6 4.0 0 0 5.6 60 min 5.6 3.8 0 0 5.6 120 min 5.6 2.5 0 0 5.6 180 mm 5.4 1.5 0 0 5.4

Table 4: Antiviral effect of essential oil on ORF virus titer by pock reduction test. (Table 4) showed clearly that the Orf virus isolate was held at 37°C, a 1.1 Log units decrease in the titer was evidenced by the 30 minutes, then, gradual decreasing until complete inactivation. When the viral isolate was held at 56°C, the decrease in viral activity was much accelerated, with a bulk of the virus 3.4 Log units being inactivated within the first twenty minutes and the complete inactivation was achieved within 30 minutes. Essential oil mixed with virus suspension (v/v) decrease viral activity 3.1 Log units complete inactivation was achieved within 40 minutes.

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