| Name |
Primer sequence |
Location |
Purpose |
| RAVGF* |
5′ CAAGGAAAGATGGTTCCTCAG 3′ |
3309-3329 |
RT-PCR |
| RAVGR |
5′ TCACAGTCTGGTCTCACCTCCAC 3′ |
4870-4892 |
RT-PCR |
| GS3* |
5′ GTACCCGGACTACCACTGGCTCAG 3′ |
3721-3744 |
Sequencing |
| GS4* |
5′ GGAACGTGGGTTGCGATGCAAACA 3′ |
4088-4111 |
Sequencing |
| GS5* |
5′ GGTATAATACTGGGTCCTGACGGC 3′ |
4460-4483 |
Sequencing |
| M13 F* |
5′ GTAAAACGACGGCCAG 3′ |
|
Sequencing |
| M13 R |
5′ CAGGAAACAGCTATGAC 3′ |
|
Sequencing |
| PSTPK5* |
5′ CTTCAACTGCAGATGGTTCCTCAGGTTCTTTTG 3′ |
|
Pst1 site |
| KPNPK3 |
5′ GCCCTAGGTACCTCAGAGTCTGGTCTCACCTCC 3′ |
|
Kpn1 site |
| PFG5 |
5′ GATGCTCATTACAAGTCAGTCGAGACCTGGAATGAGATCATC 3′ |
|
Mutagenesis |
| PKG3 |
5′ GACTGACTTGTAATGAGCATCTGCTTCCATCAAGGT 3′ |
|
Mutagenesis |
