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Mobile phase: water:methanol:ammonium sulphate (1:1:4 v/v/v). RF 0.37±0.02 for Metformin, RF 0.59±0.02 for Atorvastatin, RF 0.75±0.02 for Glimepiride Concentration of drugs: 100 μg/mL of MET, GLM and ATV Application volume: 10 μL. |
Figure 4: Densitogram of standard values. |