| The target molecule Ci to be identified is adsorbed by the stationary phase
(e.g. silica gel column). Other coexisting molecules designated as Cj (j =2,
3, 4, 6, 6, 7, ….., j) are interacting with this centered molecule Ci in different
degree of force. The intermolecular action depends on each individual solubility
parameter δi and δj (j =2, 3, 4, 6, 6, 7, ….., j). The resultant vector sum can
be either repelling (upper) or dragging (lower). In a system of infinitely dilution
these intermolecular forces can be neglected. As can be expected, the higher
concentration of sample would exhibit higher degree of interaction to result in
more serious shit of the retention time. |
