| BCPN standard (96% in purity) was used as the reference standard. BCPN (a:
5 ppm, b: 10 ppm, c: 20 ppm, d: 50 ppm, and e: 100 ppm) were reacted with anthryl
diazomethane (ADAM) to form fluorescence derivative. The mobile phase
used was methanol: water (=80:20 v/v) operated at 0.6 mL/min. The fluorescence
was taken with a fluorescence detector (FL type L-2480) at λEc = 365 nm;
and λEm=430 nm. The arrows indicate the retention time of the BCPN derivatives.
The correlation equation obtained was Y=266716x+2E+6 (R2=0.9441),
where Y was the peak area and x was the concentration of BCPN (ppm). |
