HPLC Analysis
Sample Area Retention timeb
(min)
Authentic BCPN, ppm
  5
  10
  20
  50
  100
-
176026±185
6605644±1239
10790905±985
14311427±198
28009844±251
-
28.48±0.15D
27.59±0.08A
27.43±0.11A
28.00±0.09B
28.32±0.15C
Urinary BCPN 5253622±1023 28.23±0.11C
 
GC/MS Analysis
Authentic BCPN - 18.00±0.11B
Urinary BCPN - 17.30±0.09A
aTreated as described in section “Materials and methods” of the text.
bDetermination was performed in five replicates.
Different upper case superscripts indicate significant deviation (p<0.05) in either
HPLC or GC/MS analysis.
Table 1: Comparison of the retention times between the standard BCPN and the urinary BCPN analyzed by HPLC and GC/MSa.
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