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| Figure 4: HPLC chromatogram of SS and TP obtained from degradation studies: acid hydrolysis (0.1 M HCl, 80°C, 24 h) (A); alkaline hydrolysis (0.1 M NaOH, 80°C, 24 h) (B); neutral hydrolysis (water, 80°C, 24 h) (C); oxidative degradation (3% H2O2, RT, 48 h) (D); thermal degradation (dry heat, 80°C, 48 h) (E); and photolytic degradation (photostability chamber, 30 d) (F). Chromatographic conditions: Phenomenex C18 column (250 mm × 8 mm i.d., particle size 10 µm); flow rate 1.2 mL min-1; mobile phase acetonitrile and phosphate buffer 65:35 (v/v) (pH 4.2 ± 0.02, adjusted with triethylamine) and UV detection at 235 nm. |
