Parameter TMS FIN
Linearity range (ng/ spot) 200 – 1200 1000 – 6000
Correlation coefficient 0.9991 0.9987
Limit of detection (ng/spot) 17.47006 61.98041
Limit of quantitation (ng/spot) 52.93956 187.3935
% Recovery (n = 9) 99.77 99.75
Ruggedness (% R.S.D.)
Analyst I ( n = 3) 1.19 0.95
Analyst II ( n =3) 0.71 0.71
Precision (%R.S.D.)    
Repeatability of application (n = 6) 1.04 0.73
Intra-day (n = 3) 0.65 – 1.86 0.41 -0.68
Inter-day (n = 3) 1.04 – 1.70 0.58 – 1.02
Robustness Robust Robust
Specificity Specific Specific
Table 3: Summary of validation parameter.
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