| Intra-day variation (six replicates at each concentration) |
| Theoretical concentration
(ng/mL) |
Run |
Measured concentration (ng/mL) |
| Mean |
SD |
RSD |
Accuracy % |
| 0.50 |
1 |
0.53 |
0.01 |
1.89 |
116 |
| 2 |
0.59 |
0.08 |
13.6 |
105 |
| 3 |
0.49 |
0.09 |
18.4 |
98.2 |
| 4 |
0.55 |
0.05 |
9.09 |
103 |
| 1.50 |
1 |
1.48 |
0.03 |
2.03 |
96.5 |
| 2 |
1.57 |
0.07 |
4.46 |
104 |
| 3 |
1.53 |
0.06 |
3.92 |
102 |
| 4 |
1.61 |
0.02 |
1.24 |
112 |
| 500 |
1 |
498 |
2.51 |
0.50 |
98.5 |
| 2 |
508 |
2.14 |
0.42 |
107 |
| 3 |
512 |
5.41 |
1.06 |
101 |
| 4 |
507 |
6.97 |
1.37 |
92.6 |
| 800 |
1 |
821 |
5.90 |
0.72 |
107 |
| 2 |
805 |
3.85 |
0.48 |
97.6 |
| 3 |
816 |
8.21 |
1.01 |
105 |
| 4 |
829 |
7.31 |
0.88 |
113 |
| Inter-day variation (twenty four replicates at each concentration) |
| Theoretical concentration
(ng/mL) |
Mean |
SD |
RSD |
Accuracy % |
| 0.50 |
0.53 |
0.09 |
17.0 |
104 |
| 1.50 |
1.51 |
0.08 |
5.30 |
98.7 |
| 500 |
512 |
6.71 |
1.31 |
101 |
| 800 |
823 |
9.87 |
1.20 |
109 |
RSD, Relative standard deviation (SD × 100/mean).