| Nominal concentration
(ng/mL) |
Stability |
Mean ± SD, n = 6 (ng/mL) |
Accuracy (%) |
Precision (%CV) |
| 1.50 |
0 h |
1.51 ± 0.03 |
98.2 |
3.52 |
| Third freeze–thaw |
1.60 ± 0.05 |
108 |
3.38 |
| 6 h (bench-top) |
1.45 ± 0.06 |
93.5 |
4.15 |
| 12 h (in-injector) |
1.41 ± 0.04 |
90.2 |
2.57 |
| 30 days at −80°C |
1.55 ± 0.02 |
95.2 |
3.87 |
| 800 |
0 h |
804± 5.54 |
114 |
8.74 |
| Third freeze–thaw |
795± 10.5 |
97.5 |
4.58 |
| 6 h (bench-top) |
813± 7.81 |
103 |
2.34 |
| 12 h (in-injector) |
805± 5.78 |
98.7 |
4.08 |
| 30 days at −80°C |
785± 7.87 |
101 |
4.62 |
|
