Method Added amount
(mg/mL)
Founda amount (%) Recoverya (%) + R.S.D.
HPLC 240.00 242.33 100.97 + 0.52
360.00 360.86 100.24 + 0.43
480.00 487.01 101.47 + 0.26
600.00 610.14 101.69 + 0.34
900.00 907.74 100.86 + 0.66
1200.00 1216.44 101.37 + 0.45
Bioassay 25.00 24.24 96.95 + 6.66
50.00 48.48 96.96 + 6.13
100.00 97.98 97.98 + 5.15
aMean of three replicates
Table 2: Recovery data of standard solutions added to the placebo analyzed by using the proposed LC and bioassay methods
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