Figure 2:
a) TNFα saturation curve with optimized assay parameters (0.25 μM sTNFR
1
, 0.25 mM biotin-anti- TNFα, and 2.5 μM streptavidin-HRP). Control is with no TNFR
1
; b) Suramin inhibition curve with 7 nM TNFα, IC
50
= 0.39 mM, R
2
= 0.90
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