Effect Flow Rate Variation on Assay (ng/mL)
Flow Rate (mL/min)
Change Level
100 ng/mL
250 ng/mL
500 ng/mL
0.9
-0.1
101
243
501
1.0
0
98
249
505
1.1
+0.1
97
253
499
Avg ± SD
99 ± 2.08
258 ± 5.03
501 ± 3.06
CV (%)
2.1
2.0
0.6
Table 4:
Assay results of three samples with known concentrations under different flow rates.
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