Effect Flow Rate Variation on Assay (ng/mL)
Flow Rate (mL/min) Change Level 100 ng/mL 250 ng/mL 500 ng/mL
0.9 -0.1 101 243 501
1.0 0 98 249 505
1.1 +0.1 97 253 499
Avg ± SD   99 ± 2.08 258 ± 5.03 501 ± 3.06
CV (%)   2.1 2.0 0.6
Table 4: Assay results of three samples with known concentrations under different flow rates.
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