Drug tR K’ N T Rs α
Isosbestic point
ROS 3.02 3.14 2937 2.19 - 1.09
ALP 3.69 4.06 3355 1.77 2.80 1.29
DCL 5.19 6.11 4260 1.43 0.24 1.19
Time program method
ROS 3.03 2.32 3166 - 0.21 1.49
ALP 3.74 3.07 3472 1.24 2.90 1.32
DCL 5.31 4.77 4350 0.96 0.32 1.14
Retention time (tR), Capacity factors (k’), Theoretical plates (N), Tailing factor (T), Resolution (Rs), Separation factor (α)
Table 1: System suitability parameters.
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