Drug Linearityµg mL-1 Intercept Slope R2 SEa SEEb LODng mL-1 LOQng mL-1 Isobestic point ROS 0.02-0.64 37209 385689 0.9980 0.0083 0.0117 4 12 ALP 0.125-4.0 28678 82038 0.9986 0.0608 0.0942 17 52 DCL 0.05-1.60 18236 229464 0.9982 0.0169 0.0277 7 22 Time program ROS 0.02-0.64 33408 428502 0.9992 0.0050 0.0074 3 9 ALP 0.05-1.60 45080 168733 0.9986 0.0471 0.0668 6 19 DCL 0.02-0.64 27893 553467 0.9984 0.0115 0.0180 1 3 Serum ROS - 34415 393904 0.9999 0.0053 0.0054 3 9 ALP - 24418 83294 0.9999 0.0282 0.0311 13 40 DCL - 17455 227581 0.9999 0.0110 0.0125 10 29 a=standard error estimate, b= standard error

Table 2: Regression characteristics and sensitivity of the method.

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